The novel coronaviruses belong to the B genus. COVID-19 is an acute respiratory infectious disease. People are generally susceptible. Currently, the patients infected by the novel coronavirus are the main source ofinfection; asymptomatic infected people can also be an infectious source. Based on the current epidemiological investigation, the incubation period is 1 to 14 days, mostly 3 to 7 days. The main manifestations include fever, fatigue and dry cough. Nasal congestion, runny nose, sore throat, myalgia and diarrhea are found in a few cases. This kit is used for in vitro qualitative detection of novel coronavirus 2019-nCov in respiratory specimens including oropharyngeal swab, sputum, bronchoalveolar lavage fluid and nasopharyngeal swab. Primer sets and FAM labeled probe are designed for specific detection of ORFlab gene of 2019-nCov. VIC labeled probe for N gene of 2019-nCov. Human RNase P gene extracted concurrently with the test sample provides an internal control to validate nucleic extraction procedure and reagent integrity. Probe targeting human RNase P gene is labeled with CY5. Components 50 tests/kit RT-PCR Reaction Mix Reagent 240μL ×1 tube Enzyme Mix Reagent 72μL ×1 tube 2019-nCoV primer probe 48μL ×1 tube Positive control 200μL ×1 tube Negative control 200μL ×1 tube Sensitivity: 200 copies/mL. Specificity: No cross reaction with SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63 and HIN1, H3N2, H5N1, H7N9, Influenza B, Parainfluenza Virus (123), Rhinovirus(A,B,C), Adenovirus (1,2,3,4,5,7,55), Human interstitial pneumovirus, Human metapneumovirus, EBv, Measles virus, Human cytomegalic virus, Rota virus, Norovirus, Mumps virus, Varicella Zoster Virus, Mycoplasma pneumonia, Chlamydia pneumonia, Legionella, Bordetella pertussis, Haemophilus influenza, Staplhylococcus Aureus, Streptococcus Pneumonia, Streptococcus pyogenes, Klebsiella pneumonia, Tuberculous bacillus, Aspergillus fumigatus, Candida albicans, Candida glabrata, Cryptococcus neoformans. Precision: CV ≤5%. Real-Time PCR System: Aehealth Diagenex AL, ABI 7500, ViiATM 7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0. Bio-Rad CEX96 TouchTM SLAN-96S.SLAN-96P Environment and Manufacturing Equipment Our factory has a clean workshop of 10,000 square meters, the core manufacturing equipment is imported from Germany, and 5 R&D centers have been set up all over the country. Strong R&D strength Our R&D center accounts for 40% of all employees of the company, 70% of all employees have a bachelor degree or above, and 30% have a master degree or above. Core Raw Material Through independent research and development, important technologies such as genetic engineering technology, single/polyclonal antibody preparation technology, and small molecule total synthesis technology has been mastered, which can be used to independently produce some of the required bioactive materials, chromatographic media, controls, calibrators and other common used raw materials. Quality Assurance The company's Strict quality control procedures and quality management system, production process standardization, finished product inspection, supervision and inspection,strict control of key processes.