This kit is used for in vitro qualitative nucleic acid detection of 2019-Novel Coronavirus (2019-nCoV), Influenza A and Influenza B simultaneously in respiratory specimens including oropharyngeal swabs and sputum. Primer sets and FAM labeled probes are designed for specific detection of both ORFlab and N genes of 2019-nCoV, VIC labeled probe for human RNase P gene as internal control, ROX labeled probe for M gene of Influenza A, CY5 labeled  probe for  NS gene of Influenza B. Components 50 tests/kit RT-PCR reaction buffer 915 μL ×1 tube RT-PCR enzyme mix 75 μL ×1 tube Positive control 100 μL × 1 tube Negative control 100 μL × 1 tube Sensitivity：200 copies/ mL. Specificity：Specificity：No cross reaction with SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63,Parainfluenza Virus, Human Adenovirus, Human Rhinovirus, Respiratory Syncytial Virus (A), Respiratory Syncytial Virus (B), Enterovirus A,Staphylococcus Aureus,Streptococcus Pneumonia,Klebsiella Pneumonia. Precision： CV ≤ 5%. Real-Time PCR System: Aehealth Diagenex AL, Molarray MA-6000, ABI 7500, ViiATM 7, QuantStudio 7 flex, Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX96 TouchTM/iQTM 5，SLAN-96S/96P