This kit is used for in vitro qualitative nucleic acid detection of Monkeypox Virus in Human serum, lesion exudate samples and scab specimens. Primer sets and FAM labeled probes are designed for specific detection of Monkeypox Virus, Human RNase P gene extracted concurrently with the test sample provides an internal control to validate nucleic extraction procedure and reagent integrity. Probe targeting human RNase P gene is labeled with VIC. Components 48 Tests/kit 96 Tests/kit PCR reaction buffer 672 μL × 1 tube 672 μL × 2 tube PCR enzyme mix 50 μL × 1 tube 100 μL × 1 tube Positive control 100 μL × 1 tube 200 μL × 1 tube Negative control 100 μL × 1 tube 200 μL × 1 tube   1. Sensitivity: 200 copies/mL. 2. Specificity: No cross reaction with Enterovirus(EV), Measles virus(MV), Rubella virus(RV), Varicella-zoster virus(VZV), Dengue virus(DenV), Human Parvovirus B19(HPVB19), Epstein-barr virus (EBv), human herpes virus 6(HHV-6) 3. Precision: CV ≤ 5%. Real-Time PCR System: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX96 TouchTM/iQTM 5，Hongshi SLAN-96S/96P，AGS8830, AGS4800