High-Performance Liquid Chromatography (HPLC)

High-performance liquid phase analyzer (HPLC) is a kind of mobile phase that uses liquid as the mobile phase. The sample and solvent are transported to the chromatographic column filled with the stationary phase through the high-pressure pump. According to the different interaction forces between the different components in the sample and the stationary phase, the Chromatic techniques for the separation, qualitative and quantitative analysis of samples. It has the advantages of high separation efficiency, fast analysis speed, high sensitivity and good reproducibility, and is widely used in organic chemistry, biochemistry, medicine, food, environment and other fields.
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The Laffinite II Haemoglobin A1c method utilises the principles of ion exchange high performance liquid chromatography (HPLC) for the automatic separation of Haemoglobin A1c (HbA1c). Whole blood is sampled directly from the primary tube, diluted in haemolysing buffer and pumped via a pre-filter to the ion-exchange column.

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